It is based on the observation that rats and mice will bury either harmful or harmless objects in their bedding.
Marble burying test autism.
The marble burying test takes advantage of the proclivity of mice to dig in natural settings e g.
Marble burying test and self grooming behavior.
Burrows escape tunnels and in standard cage bedding and the nestlet shred test capitalizes on the fact that mice are nesting animals by nature that build nests for protection of themselves and their offspring against environmental conditions e g.
When put in a cage with marbles mice with ocd like symptoms tend to engage in a high degree of repetitive behaviors including digging while mice with a high degree of anxiety tend to engage in a high degree of digging in novel contexts potentially as a means to attempt.
Subjects received a different treatment before marble burying from that administered during the spontaneous self grooming experiment with the exception of three b6 mice.
The marble burying test protocol described below is modiļ¬ed from deacon 2006 and angoa perez kane.
For the repetitive domain the t maze spontaneous alternation test and marble burying test are described.
The marble burying test occurred 8 days following the grooming test to ensure there were no potential residual effects from the initial drug treatment birdsall et al 1978.
If a transparent ceiling with breathing holes is placed on top of testing cage this will prevent the test subject from leaving the cage while allowing unrestricted video recording.
The marble burying test in animal models of asd such as the btbr t tf j mouse eif4e transgenic mice 4e tg as well as in the maternal immune activation mouse model amodeo et al 2012.
The setup for the marble burying test consists of a standard mouse housing cage filled with a thick layer 5 cm of shaved aspen bedding.
In the current study significant differences in marble burying behavior were observed between pm 2 5 exposed and control rats figure 1b and supplementary figure 3 f 3 35 37 56 p 001.
For the communication domain neonatal ultrasonic vocalization and olfactory habituation test toward social and non social odor are described.
Choi et al 2016.
Marble burying is an animal model used in scientific research to depict anxiety or obsessive compulsive disorder ocd behavior.
Santini et al 2013.
The marble burying test is a useful model of both obsessive compulsive like ocd behavior and or anxiety like behavior.
In this unit behavioral tests are described that are relevant to the domains affected in asd.
The number of marbles that were buried by the mouse was measured.
